A very generous member gave me some excess rooted cuttings and I am transferring them to cups this evening. Can someone give me an idea on how much water they use in the initial watering once they transfer rooted cuttings to cups? Thanks!

Me personally, NONE. My 50/50 soil/perlite is damp from days way before potting up but I add no water. Just a vented cup on
top.

It really depends on how moist your soil is to begin with and what is the size of your cup or pot. Then you just gotta kinda have a feel for it, you want it damp not soaking. This is a good reason to use perlite or some other soil amendment that will allow air into your soil  

Thanks Mike and Barry. My soil/perlite mix was very dry and I needed some but wasnt sure how much.

I have been using Perlite/MG Seed Starting mix with my cuttings in a 70/30 or 80/20 proportion. Potting soil and mixes are not sterile and are usually inoculated with mold and fungus spores unless they are sterilized. Once cupped, temperatures should be maintained about 75 deg F for fast root growth, at least for the first few weeks. I usually water the mix and let it drain, before potting the cutting and never "water in" just tap lightly on bottom of cup. Too much moisture will always cause root rot.

I use the 70/30 perlite/soil mix in the cup.  I soak the mix with warm water until it just starts to drip from the bottom.  So far, good success.  Not saying it's the best way, just my 2 cents.

The soil/perlite mix that I use is only 'slightly' damp, so I give my transplanted cuttings a good shot with the spray bottle, just to water them in.  After that I treat them like cactus plants (just a spoonful of water now and then.)  I have not had any issues with root rot or mold using this method.

I use about a 50/50 mix of UPM potting mix and perlite.  I add just enough water in the mixing container so that it barely hold together in your hand.  I then put a dome cup on top and only add water if it gets very dry and then just a little - 2-3 tablespoons per cup max.  Once they get leaves, the dome comes off and I spritz the leaves every evening with a spray bottle I got at HD.  Excessive water is your enemy...you will rot the cutting and roots with too much.  Remember, until you get leaves, you only need enough moisture to get the cutting viable.  Like ForeverFigs said, treat it like a cactus to start with.  Too little will not kill them...too much and they are doomed.

What about only 60% perlite an 40% vermiculite?

Is it better to mix perlite with potting soil for the rootingdevelopment in cups?

The answer to all of these questions are: "It depends"  Read the rooting section here

http://figs4fun.com/basics_Intro.html

and learn the principles.  If your perlite has a lot of dust all the air spaces will be clogged and filled with water.  Your cutting will rot and die.  Observe what you have and see if you have moist air spaces.  If not, then create them.

I went back to all my cups and burned holes into the cups to get more air to the roots. I think some of my roots could of done better if they had more air. Having some success finally rooting. I think once you find a good mix of Soil, Perlite and Potting mix this will help. We learn from our mistakes. Good Luck.!!!