Partial excerpt from fig scion experiment i had saved with other files .
There is small rooting percentage for the
production of cuttings under normal field conditions. This is caused by various factors,
including the rooting environment and the growth regulator endogenous content, whose
effect will be reflected upon the cultures productivity and quality. The cutting's rooting is
regulated by a number of factors, whose lack can limit the process. The tissues capability
of forming roots depends on various endogenous and/or exogenous factors and their
interaction (Nementh, 1986; Haissig, 1982; Thompson and Thorpe, 1987). Auxins are
part of a growth regulators group having a great effectiveness on inducing rooting. IBA is
the most commonly used synthetic auxin for the adventitious rooting induction and is
mentioned by many authors, as being a rooting promoter (Wang and Andersen, 1988). It
has been used to induce rooting on many plant species due to its property of causing
primary roots formation (Awad and Castro, 1989). An auxin exogenous application
produced roots in various species (Sriskandarajah and Mullins, 1981). The use of
artificial substrates has been currently used in agricultural production as a means for
rooting, growth and plant production (Bunt, 1983; Gabriels et al., 1986).
There are many
types of different substrates available, as for example, sawdust, sand, soil, carbonized rice
straw, vermiculite, and many others, making it difficult to choose their best one and the
best mixture (Blom, 1983; Gabriels et al., 1986; Chen et al., 1988). Each material has its
own physical and chemical characteristics and a specific use (Bunt, 1971). A rooting
substrate must be of low density, have a fair water retention capacity, aeration and
drainage, also both have a good cohesion between the particles or close to the roots and
finally, it must be preferably sterile (Mello, 1989). Stratification is a technique that
consists of lying alternate layers of rough sand or soil. The cuttings must be removed
from the stratification as soon as the callus and/or buds have been sprouted (Fachinello et
al., 1994).