for two healthy looking plants to all of a suddenly wilt? Not too wet or dry. I have taken both out of their pots and check for gnat larve and found none. The Black Madeira on the top and Qudssaya on the bottom. They both had one leaf to curl and fall off.

Add humidity.

get a spray bottle and mist em. i mist all mine a few times daily

Jon, the grown room is 72 degrees with humidity at 58% and the other 31 plants with leaves look fine. What humidity level would you recommend?

Not telling you to do this but... If I suspect any problems I just pull out and re do in Moss if roots look withered and bad.

Good idea Dominick. The roots looked fine so I just repotted.

The worst I have experienced from a one-day-healthy-rooting, and then suddenly comes the leaf-drooping,
was that the main-twig just plain rotted, thereby breaking the plumbing connection between the roots and the leaves.
I did manage to salvage a little-few by adding more potting soil on top, slightly above the green shoot,
and covering with a (vented) dome. Too much water usually causes rot.

However, it may also be a lack of water/moisture as others suggested.

You alone know the details and be the judge.

Good luck.

I keep all my cups in humidity bin. Unless they are harden, they will wilt once out of the bin. My house is very dry.

Humidity of 90% or more will fix the problem. Next time when you take them out your bins reduce humidity slowly over several days. 5% a day till you reach ambient humidity.

Mike where you buy your perlite, looks good and coarse.

Eden, this is where I get mine. I can't find the large size anywhere else around here. I use the #3 which averages 1/2". The #4 averages 1". The store is I go to is off Old National Hwy close to the airport.

1422 Woodmont Lane #4
Atlanta, GA 30318
404.367.0052

Atlanta, GA: From downtown Atlanta, take 75 N, exit Howell Mill Road (#252B) and go left. Go 5 lights and turn right onto Chattahoochee Ave. (continue on Chattahoochee for exactly 1 mile). Turn left on Woodmont Lane and Atlantis Hydroponics™ is on the left, in Suite 4.

2561 West Point Ave
College Park, GA 30337
678.510.0032

College Park, GA: From Atlanta, take 75/85 S until the split, then take 85 S, cross Int. 285 and exit Old National Hwy (#69). Turn right, then right again at the first light onto Sullivan. Immediately go left onto West Point Ave. Atlantis Hydroponics™ is the first building on the left.

5182-B Brook Hollow Pkwy
Norcross, GA 30071
770.558.1346

Norcross, GA: From Atlanta, take 85 N past Int. 285 and exit Indian Trail Road (#101). Turn left, cross over 85, then left again at the first light onto Brook Hollow Pkwy. Atlantis Hydroponics™ is on the left in the Brook Hollow Center.

From North of Atlanta, take 85 S and exit Indian Trail Road (#101). The exit will end at Indian Trail Road. Cross over Indian Trail Road to Brook Hollow Pkwy. Atlantis Hydroponics™ is on the left in the Brook Hollow Center.

Thank you Mike I just call Norcross office and will go there tomorrow.

Eden, they have a lot of stuff we use such as pots, pro-mix and  BT.

Did you add fertilzer? Even if you diluted fertilzer that was NOT diluted enough, this happens, sometimes it will burn the roots if the fertilzer is too strong. If it is the case, I am sure you flushed it out.

If it not fertilzer, then wood rot may be the culprit. So many scenerios. Just leave it alone and mist. Majority of the time I see this will likely die. Sorry to be a "negative Nancy" but this happened to me from my own experience (on several attempts to revive my cuttings). 90% of the time is "human error", dont smother it silly.. That's how we drown it! -- me in particular, not you

Jennifer

Thanks Jennifer. No fertilizer.  I am upping the humidity and doing some misting.

Mike sometimes it happens for various reasons mentioned but will can be fine eventually without much intervention.
Leaf drop i have seen happen many times only to have a bud eventually open and thrive and i see one on yours  - keep your fingers crossed and good luck.

Thanks Martin. Of course this is one of my prizes (to me) and I got a little anxious about it. You ever done that?

LOL Mike oh yeah !
Then last time i rooted anything the knats took care of them, i had ordered some from
Jon and R.Watts and that broke me to the point of never rooting anything anymore indoors.

The point of humidity is this: roots take in water, leaves lose (transpire) water. The higher the humidity, the harder it is for the leaves to lose water. If you lose less water, you need to take in less water. With newly rooted cuttings, the shoots and leaves may be losing water faster than the new, tender roots can take it in. It isn't just about how much you water or how wet the soil is, if the roots cannot take the water in, for whatever of several reasons.

if your cuttings were in a hu8mid environment, were repotted and then placed in a less humid environment, you have changed the dynamics of the water-in/water-out balance. If you damaged even some of the roots when repotting (or from baggie to cup) then you have also upset the balance.

There are other issues, such as mold, rotting of the roots, etc. that can also be an issue or cause but managing humidity is a fundamental issue.

Mike,

Along Jon's comments above, I kinda sorta harden off my cuttings when going from the closed, heated Sterlite container to a south facing window sill.  I put my deli containers in a much smaller Sterlite container (no added heat), add about a half teaspoon of water to the Sterlite container, close/open the lid on alternating days, place in a south facing window and wait 4-7 days before placing the deli container on the window sill.

Steve good plan. Unfortunately I don't have the south window without rearranging my wife's
sunroom and she wouldn't go for it anyway since I have a 10x20 grow room. Dealing with the
number of cups is another issue too. LOL