Again sorry its a link to another forum i posted on. i have had alot of success with microproagation but have never been able to culture figs. if anybody know how please share. and pics would be nice.

dave

link
http://www.gardeningclub.com/community/forums/aft/40643

Springerlink has a lot of articles for free for the holiday season.  There are a few on micropropagation of figs.  Download the PDFs.  They'll be back to $30 each soon. Check and see if your nearest university library has Protocols for Micropropagation of Woody Trees and Fruits  2007.  Chapter 37 is on figs (PP 409-416)

Thanks Bob, i will deffenatly get right on that. there is so much stuff out the its hard to find exactly what your looking for sifting through thousands of pages. If the library has it and i like i might just have to buy a copy. thanx again.

Dave

The U should give you guest access to print those 8 pages.  The book on CD is over $200.  On springerlink just search for micropropagation ficus carica.

"The reference manual for woody plant propagation," Dirr and Heuser, has a chapter on tissue culturing.  It offered at the AHS and I think Amazon,  far cheaper than the expensive college reference book and the basics at the right level for small use use or a try. I have done it three times, twice with success. My thoughts to date is it is worth the mess and time for a special or rare one where most stock I have access to is contaminated with FMV.

Happy Thanksgiving folks, and safe travels

That's a great book but different trees need different protocols   The pages referenced give specific protocols that work for our fig trees.  They are complementary, though.  The Reference Manual has great info on setting up a tissue culture lab which is assumed as already done by Protocols.

Yes, I have both books, however, for those wishing to read the basics, was trying to state other options are there for those not wishing to spend $200.

I have cultured blackberries and other bramble fruits, and bananas fairly well but never had luck with figs as of yet. any info in regards that can get me to that point would make me happier than beaver in a saw mill.

Sorry if I wasn't Clear.  No one interested in only figs should buy a $200 CD that only has 8 pages on figs.  Your friendly neighborhood tax supported university should be able to help you print the 8 pages of interest for about $1. 

The Reference Manual has great info on grafting and other propagation techniques as well as an introduction to setting up a lab and prepping the specimen.  A cheaper book, coming out in 4th edition this Spring is Plants from Test Tubes: An Introduction to Micropropagation.  It's only about setting up a lab and has some sample protocols.  No info on grafting or other types of propagation.

Even cheaper is to watch some of the videos on youtube    :)

Dave, go here  The first 2 articles are on fig micropropagation.  click on d/l PDF and they'll appear on your screen.  You can save them to your hard drive.  After this holiday promotion is over it will cost a lot of $$ to do that so do it soon.  For the book, take the info I gave you to your nearest tax supported university and they'll help you get the pages you need.

Thank you got it downloaded. i want to cultur various things and have done a few. just havent had any luck with figs

Dave (or anyone else who got this downloaded),
Can you send me a pdf of chapter 37 of Protocols (pp 409 - 416)?  I just went to download it... they've taken away the free access already (just let you read the first 2 pages, but pay to see more).  Stupid me to have waited to download.  I did download a couple of other articles (e.g. the Fraguas et al, 2004 paper).

Mike

Dave, are you a member of the HTC group?  Folks are usually very helpful there although I see someone else posted the same question over there in October and didn't get a reply.  There are some professionals and very experienced hobbyists in the group that have come to help in the past.  One member heads up the lab for a farming company with about 72,000 acres of tree crops here in California.

http://tech.groups.yahoo.com/group/hometissueculture/message/30615

Maybe you could bump this post.  Good luck.

no harvey, but it looks like a group i should get in with. i am trying to get my ducks lined up and go back to school. see about taking my generals online to at least get them out of the way.

I recently bought a Blue Ischia fig from Hirtsgardens.
http://www.ebay.com/itm/290782329328?ssPageName=STRK:MEWNX:IT&_trksid=p3984.m1497.l2649

It is a puny/small (but healthy) 4" plant with a 1/8" stem and 1" leaves  ... and sooo ...  it most likely was reproduced from tissue culture?

Also, it may be the (infamous/good) Ischia Black, which any twig-rooting notoriously maintains its severe FMV infection.

I'll wait/experiment/see for myself ... so far, it seems to be FMV free ...

Yeah, but what we really want to see is if it's a Black Ischia or another name for brown turkey.  :)

Mike, the book pages were never available for free online.  You have to get them from your local University.  It should be very inexpensive there.

And the guy who got no answer in Oct?  That was me  :)

Actually they are available online:

http://diyhpl.us/~bryan/papers2/bio/agrobacterium/superkuh.com/library/Biology/Agrobacterium/Protocols%20for%20Micropropagation%20of%20Woody%20Trees%20and%20Fruits_%20S%20Mohan%20Jain_%20H%20Haggman_%202007.pdf

Large file so make sure you have a high speed connection

Clearly the blog site of a biological student or researcher:  beware of copyright laws before you attept to distribute or sell. for educational download use only.

http://diyhpl.us/~bryan/papers2/bio/agrobacterium/superkuh.com/library/Biology/Agrobacterium/

Thanks for the reference links.  I've begun reading Protocols... been interested in this topic (not sure if I'll try anything with it yet, but interested).  

Mike

Duarte Nursery does tissue culture of many different trees and vines and I toured their facilities a few years ago.  I met the Director of Research that heads up the TC lab, Javier Castillon.  You might try emailing him to see if he might help. first name and then @duartenursery.com according to his business card.  I'm curious what varieties they're interested in working on.

Strong work, Jack!

After reading a post about this fig I broke down and purchased one too out of curiosity.  I thought it looked like an elm tree seedling when it arrived.  Can anyone explain why a tissue culture grows differently than a cutting initially?  Does a Blue Ischia variety exist?  I wonder where this name came from?  I wonder if they would be interested in trying to produce FMV free Ischia Black varieties if tissue culture techniques can eliminate it?

That is great progress.  Do yo have the mini greenhouse inside?  I haven't given mine any special attention yet but having seen your progress I think I'll put some more effort into getting it to grow inside.  I thought mine looked like an elm tree seedling out of the box.

Quote:

Originally Posted by SCfigFanatic
I bought the same plant from the same source.
The first picture was on 10/5/12 when I got it.
The second picture was from today. I pulled it from my mini greenhouse
with 14 hours of light daily.
Not trying to help seller of this fig, it looks nothing like
they advertise by their picture.

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It does have leaf rust....

Doug

I have to ask:  

• Why (and how) can tissue culture propagation techniques result in a tree that is free of FMV, if the initial source of the tissue sample is a tree with FMV?
• Wouldn't the tissue culture propagate the FMV viruses, just as it propagates the tree itself?  
• Or is there something in the process that kills the viruses but doesn't kill the tree tissue?  
• Or are you assuming that it's possible to to get a small sample of virus-free tissue from an FMV-infected tree? 

I don't know anything about these tissue culture techniques either but I seem to recall a discussion that new fast growing growth might not yet have the FMV in the cells.  I think it takes something like an electron microscope to see a virus.  A FMV free tree might stay free of the virus longer in the North East but would have a good chance of getting infected again in warmer regions.  Does UC Davis conduct research on FMV?

Citrus are supposedly cleaned up of viruses by culturing the new shoots which are not yet infected.  Tissue culture methods also will sometimes treat cultures with compounds to kill viruses, I believe, though I don't know if this is always successful.

The Sierra and Sequoia varieties which I bought (Jon bought some of these from me and resold them) were bought from a nursery in Madeira, CA (nurseryman had died and wife was liquidating) that had been propagated by TC, but I don't know where or how. I only spoke with the wife briefly as life was very chaotic for her with everything that she was having to deal with.